Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate) (2024)

Sivashankar, S., Puttaswamy, S. V., Lin, L. H., Dai, T. S., Yeh, C. T., & Liu, C. H. (2013). Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate). Sensors and Actuators, B: Chemical, 176, 1081-1089. https://doi.org/10.1016/j.snb.2012.09.087

Sivashankar, Shilpa ; Puttaswamy, Srinivasu Valegerahally ; Lin, Ling Hui et al. / Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate). In: Sensors and Actuators, B: Chemical. 2013 ; Vol. 176. pp. 1081-1089.

@article{44d072447f3640728e86e55a6f446538,

title = "Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)",

abstract = "The bioreactors with an array of multiple wells favoring the maintenance of the three-dimensional (3-D) liver tissue cultures under continuous perfusion have been developed. All bioreactors were fluidically connected to each other. Each bioreactor in the array contains the poly(ethylene glycol) diacrylate (PEGDA) microstructures, cultured with the mesothelial cells that support the formation of 3-D environment. The mesothelial cells surrounding liver tissue whose primary functions in vivo are to provide a protective adhesive surface and help in tissue repair. The tissue units were continuously perfused with cell culture medium in the bioreactor. After twelve days of culture, the liver tissue surrounded by the mesothelial cells seeded in the perfused multiwell reactor remained functionally viable as assessed by H&E (hematoxylin and eosin) stain and TUNEL (Terminal deoxynucleotidyl transferase (dUTP) nick end labeling) assay examination. The liver tissue shows intact architecture and enhanced viability compared with those in conventional culture dish and incubation systems. The hepatitis B surface antigen (HBsAg) expression of the liver tissue cultured in our bioreactor was also much better when compared to the conventional static culture method. The use of primary liver sample provides more relevant experimental system and potentially replaces the animal based models.",

keywords = "Bioreactor, Hematoxylin & eosin (H&E), Poly(ethylene) glycol diacrylate (PEGDA), Terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL), Transgenic mice",

author = "Shilpa Sivashankar and Puttaswamy, {Srinivasu Valegerahally} and Lin, {Ling Hui} and Dai, {Tz Shuian} and Yeh, {Chau Ting} and Liu, {Cheng Hsien}",

year = "2013",

doi = "10.1016/j.snb.2012.09.087",

language = "英语",

volume = "176",

pages = "1081--1089",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

}

Sivashankar, S, Puttaswamy, SV, Lin, LH, Dai, TS, Yeh, CT & Liu, CH 2013, 'Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)', Sensors and Actuators, B: Chemical, vol. 176, pp. 1081-1089. https://doi.org/10.1016/j.snb.2012.09.087

Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate). / Sivashankar, Shilpa; Puttaswamy, Srinivasu Valegerahally; Lin, Ling Hui et al.
In: Sensors and Actuators, B: Chemical, Vol. 176, 2013, p. 1081-1089.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)

AU - Sivashankar, Shilpa

AU - Puttaswamy, Srinivasu Valegerahally

AU - Lin, Ling Hui

AU - Dai, Tz Shuian

AU - Yeh, Chau Ting

AU - Liu, Cheng Hsien

PY - 2013

Y1 - 2013

N2 - The bioreactors with an array of multiple wells favoring the maintenance of the three-dimensional (3-D) liver tissue cultures under continuous perfusion have been developed. All bioreactors were fluidically connected to each other. Each bioreactor in the array contains the poly(ethylene glycol) diacrylate (PEGDA) microstructures, cultured with the mesothelial cells that support the formation of 3-D environment. The mesothelial cells surrounding liver tissue whose primary functions in vivo are to provide a protective adhesive surface and help in tissue repair. The tissue units were continuously perfused with cell culture medium in the bioreactor. After twelve days of culture, the liver tissue surrounded by the mesothelial cells seeded in the perfused multiwell reactor remained functionally viable as assessed by H&E (hematoxylin and eosin) stain and TUNEL (Terminal deoxynucleotidyl transferase (dUTP) nick end labeling) assay examination. The liver tissue shows intact architecture and enhanced viability compared with those in conventional culture dish and incubation systems. The hepatitis B surface antigen (HBsAg) expression of the liver tissue cultured in our bioreactor was also much better when compared to the conventional static culture method. The use of primary liver sample provides more relevant experimental system and potentially replaces the animal based models.

AB - The bioreactors with an array of multiple wells favoring the maintenance of the three-dimensional (3-D) liver tissue cultures under continuous perfusion have been developed. All bioreactors were fluidically connected to each other. Each bioreactor in the array contains the poly(ethylene glycol) diacrylate (PEGDA) microstructures, cultured with the mesothelial cells that support the formation of 3-D environment. The mesothelial cells surrounding liver tissue whose primary functions in vivo are to provide a protective adhesive surface and help in tissue repair. The tissue units were continuously perfused with cell culture medium in the bioreactor. After twelve days of culture, the liver tissue surrounded by the mesothelial cells seeded in the perfused multiwell reactor remained functionally viable as assessed by H&E (hematoxylin and eosin) stain and TUNEL (Terminal deoxynucleotidyl transferase (dUTP) nick end labeling) assay examination. The liver tissue shows intact architecture and enhanced viability compared with those in conventional culture dish and incubation systems. The hepatitis B surface antigen (HBsAg) expression of the liver tissue cultured in our bioreactor was also much better when compared to the conventional static culture method. The use of primary liver sample provides more relevant experimental system and potentially replaces the animal based models.

KW - Bioreactor

KW - Hematoxylin & eosin (H&E)

KW - Poly(ethylene) glycol diacrylate (PEGDA)

KW - Terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL)

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=84875446469&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2012.09.087

DO - 10.1016/j.snb.2012.09.087

M3 - 文章

AN - SCOPUS:84875446469

SN - 0925-4005

VL - 176

SP - 1081

EP - 1089

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Sivashankar S, Puttaswamy SV, Lin LH, Dai TS, Yeh CT, Liu CH. Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate). Sensors and Actuators, B: Chemical. 2013;176:1081-1089. doi: 10.1016/j.snb.2012.09.087